不需要换行符时 使用file.read().splitlines()

with open("file.txt", "r") as file:
    lines = file.readlines()

print(lines)  # ['Line 1\n', 'Line 2\n', 'Line 3\n']

with open("file.txt", "r") as file:
    content = file.read().splitlines()

print(content)  # ['Line 1', 'Line 2', 'Line 3']