addgene去找你要的基因cDNA是否有现成载体
用gateway cloning把你想要的cDNA克隆到特定载体里
下游常规分析
Gateway™ LR Clonase™ II Enzyme mix
Efficient genetic editing of human intestinal organoids using ribonucleoprotein-based CRISPR
https://journals.biologists.com/dmm/article/16/10/dmm050279/330768/Efficient-genetic-editing-of-human-intestinal